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H2AX is a reported alias name for the human gene H2AFX, or 'H2A histone family member X'. The 143-amino acid protein is a member of the Histone H2A family. The cellular localization is predicted to be nuclear. Molecular weight markers in kilobase pairs are indicated on the left side of each panel. Figure 6. View large Download slide.

granzyme H (cathepsin G-like 2, protein h-CCPX) Synonyms CCP-X, CGL-2, CSP-C, CTLA1, CTSGL2. Species Human (2999) , Species domestic guinea pig (100720497) , Species naked mole-rat (101702503) , Species cow (788601) , More. Summary: The protein encoded by this gene is a member of the granzyme family. 2017-02-03 · Phosphorylated H2AX (γ-H2AX) is a sensitive marker for DNA double-strand breaks (DSBs), but the variability of H2AX expression in different cell and tissue types makes it difficult to interpret the meaning of the γ-H2AX level. Furthermore, the assays commonly used for γ-H2AX detection utilize laborious and low-throughput microscopy-based methods. We describe here an ELISA assay that 2007-01-23 · H2AX: HGNC i: HGNC:4739, H2AX: HPA i: ENSG00000188486, Tissue enriched (lymphoid) MIM i: 601772, gene: neXtProt i: NX_P16104: OpenTargets i: ENSG00000188486: PharmGKB i: PA29116: VEuPathDB i: HostDB:ENSG00000188486.3: GenAtlas i: Search Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion.

2019-08-22 · Measurement of γ-H2AX foci levels in cells provides a sensitive and reliable method for quantitation of the radiation-induced DNA damage response.

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Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post Thermo Fisher dapi Dapi, supplied by Thermo Fisher, used in various techniques.

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View specifications, prices, citations, reviews, and more. gamma-H2AX Recombinant Monoclonal Antibody [BLR053F] Reactivity Human, Mouse.

In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of core histones H2A, H2B, H3 and H4, to form chromatin. H2AX contributes to nucleosome-formation, chromatin-remodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA. The cell pellet was suspended in 25 μl of PBS (1.9 mM NaH 2 PO 4, 8.1 mM Na 2 HPO 4, 154 mM NaCl, pH 7.2), supplemented with 1 g/l bovine serum albumin (BSA), (fraction V, Sigma) and stored on ice no longer than 3 h before analysis of gamma-H2AX, or by the comet assay. I performed IF to find gamma H2AX foci in mammalian cell with different concentration of H2O2. the molecular mechanisms underlying events downstream of the D-loop formation remain unclear. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended. Electrotransfer to nitrocellulose membrane .
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Proc. Natl. Acad. Phosphorylated on Ser-136 (to form gamma-H2AX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events. 3 reviews. Compare Anti-H2AX Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

γ-H2AX can be detected by immunoblotting and immunostaining using microscopic or flow cytometric detection. Ionizing Radiation-dependent {gamma}-H2AX Focus Formation Requires Ataxia Telangiectasia Mutated and Ataxia Telangiectasia Mutated and Rad3-related. MOLECULAR BIOLOGY OF THE CELL AT1G08880 AT1G54690 AT3G48190 Invitrogen Anti-Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal (RM224), Catalog # MA5-33062. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications. This antibody reacts with All, Human, Mouse, Rat samples. Supplied as 100 µg purified antibody (1 mg/mL). 2013-11-29 · Radiation therapy is one of the most common and effective strategies used to treat cancer.
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The H2AX histone (~15 kDa calculated molecular weight) is a member of the H2A histone family whose members are components of nucleosomal histone octamers. Double-stranded breaks in DNA caused Molecular Weight: 2.016 g/mol: Computed by PubChem 2.1 (PubChem release 2019.06.18) XLogP3-AA: 0: Computed by XLogP3 3.0 (PubChem release 2019.06.18) Hydrogen Bond Donor Count: 0: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Hydrogen Bond Acceptor Count: 0: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Rotatable Bond Count: 0 The influence of gamma radiation on the molecular weight and glass transition behaviour of poly- L-lactide (PLLA) and hydroxyapatite/poly- L-lactide (HAp/PLLA) nanocomposite has been studied. Since PLLA exposed to high-energy radiation in the presence of air is prone to chain scission reactions and large degradation, changes in molecular weight were obtained by gel permeation chromatography Molecular weight and isoelectric point of various animal immunoglobulins. Immunoglobulin: approximate MW/ kDa (g·mol −1) number of subunits: pI: A280. Mg/ml) IgA The number average molecular weights (M n) of the irradiated PLLA and (polymer component in) HAp/PLLA are plotted as a function of absorbed dose in Fig. 1a.Decay in the number average molecular weight is observed at lower doses of gamma irradiation (up to 100 kGy) and followed by smaller changes at higher doses (above 100 kGy). Gamma-poly-glutamic acid (gamma-PGA) is a natural occurring, multi-functional, and biodegradable biopolymer.

Supplied as 100 µg purified antibody (1 mg/mL). Ionizing Radiation-dependent {gamma}-H2AX Focus Formation Requires Ataxia Telangiectasia Mutated and Ataxia Telangiectasia Mutated and Rad3-related. MOLECULAR BIOLOGY OF THE CELL AT1G08880 AT1G54690 AT3G48190 2006-03-17 2013-11-29 Figure 2 : Protein Structure of histone H2AX protein. Description: A single exon having a length of 432 nucleotides codes for H2AX protein of molecular weight 15 kDA. It has a characteristic histone H2A domain ranging from 18 to 91 amino acid with H2A signature motif from 22-28 aa.
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H2AX is a reported alias name for the human gene H2AFX, or 'H2A histone family member X'. The 143-amino acid protein is a member of the Histone H2A family.